Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel

Author(s)
Meder Kamalov, Hanspeter Kaehlig, Christian Rentenberger, Alexander Müllner, Herwig Peterlik, Christian F. W. Becker
Abstract

Here we show that the well-known ovalbumin epitope SIINFEKL that is routinely used to stimulate ovalbumin-specific T cells and to test new vaccine adjuvants can form a stable hydrogel. We investigate properties of this hydrogel by a range of spectroscopic and imaging techniques demonstrating that the hydrogel is stabilized by self-assembly of the peptide into nanofibres via stacking of beta-sheets. As peptide hydrogels are known to stimulate an immune response as adjuvants, the immunoactive properties of the SIINFEKL peptide may also originate from its propensity to self-assemble into a hydrogel. This finding requires a re-evaluation of this epitope in adjuvant testing.

Organisation(s)
Department of Biological Chemistry, Department of Organic Chemistry, NMR Centre, Physics of Nanostructured Materials, Dynamics of Condensed Systems
Journal
Scientific Reports
Volume
9
No. of pages
6
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-019-39148-8
Publication date
02-2019
Peer reviewed
Yes
Austrian Fields of Science 2012
Chemical biology, Biochemistry, Electron microscopy
Keywords
Portal url
https://ucris.univie.ac.at/portal/en/publications/ovalbumin-epitope-siinfekl-selfassembles-into-a-supramolecular-hydrogel(0332a6e5-1e21-4c2b-8d22-226eac7c73a7).html